Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Product Note for EVOLUTE® HYDRO Solid Phase Extraction Plates with In-Well Hydrolysis Capability
96-well plate Alcohol metabolite Amphetamines Anaesthetic Analgesics Barbiturates Benzodiazepines Carbamate hypnotic Carbamate muscle relaxant Carbamates Clinical Designer stimulants Drugs of Abuse English Forensic GC-MS Hydrolysed urine LC-MS/MS NSAIDs Opiates Opioid Opioid agonist Pain Management Pharmaceuticals Product Notes Stimulants Urine z-drugs
This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented. SOFT 2014
This poster provides mixed-mode resin-based SPE sample preparation strategies for the simultaneous extraction of EtG and EtS and was presented at EBF 2012. EtG, EtS, Poster, EBF, EBF 2012, Barcelona, LC-MS/MS, Ethyl Glucuronide, EVOLUTE, EVOLUTE EXPRESS, Polymeric, Polymer, SPE, Solid Phase Extraction,
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches. EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are specific metabolites formed within the body after the ingestion of alcohol. In forensic toxicology, when tested together they provide more specific evidence of alcohol intake. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS. Recoveries for EtG range from 90-105% and 80-103% for EtS across two formats of EVOLUTE AX columns with RSDs <10%. The LOQ is 10 ng/mL for EtG and 2 ng/mL for EtS across both EVOLUTE AX formats. EVOLUTE AX, EVOLUTE, EtS, EtG, Ethyl Glucuronide, Ethyl Sulfate, SPE, Anion Excange, Urine, OASIS, Waters, Polymeric, Polmers,