Part No: P126Issued year: 2015File size: 0.48mbFile type: pdf
This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared.
Part No: AN759Issued year: 2012File size: 1.27mbFile type: pdf
This application note describes a SPE-UHPLC-MS/MS procedure for the analysis of 8-oxodG, a biomarker of oxidative stress, from various human biofluids using ISOLUTE ENV+ solid phase extraction columns. Oxidative stress is the imbalance between the production of reactive oxygen species (ROS) and the body’s ability to eliminate them, in favour of the former. ROS have been linked with development of numerous diseases including Parkinson’s and Alzheimer’s and have also been associated with fertility problems. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is one of the mutagenic nucleobase modifications produced in DNA and the 2’deoxyribonucleotide pool, by the reaction of ROS. Measurement of 8-oxodG in biological fluids can provide a useful indicator of oxidative stress.
8-oxodG, sample prep, ENV+, urine, biological fluids, biomarker, oxidative stress, nucleotide,
Part No: AN738Issued year: 2011File size: 0.27mbFile type: pdf
The screening for elevated levels of Methylmalonic Acid (MMA) in serum is commonly used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in mammals. The significance of this screening is important for the detection of inborn metabolic disorders that can lead to the build up MMA in human biological systems. The detection and quantification of MMA can be problematic due to the abundant presence of the endogenous isobaric Succinic Acid (SA). The SA offers no clinical diagnostic value. This application note demonstrates analyte recoveries >90% with RSDs <10%.
Serum, Clinical, MMA, Methylmalonic acid, SLE, SLE+, supported liquid extraction,
Part No: P034Issued year: 2012File size: 0.48mbFile type: pdf
The screening for elevated levels of Methylmalonic Acid (MMA) in serum is commonly
used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in mammals. The significance of this screening is important for the detection of inborn metabolic disorders that can lead to the build up MMA in human biological systems
Part No: P056Issued year: 2013File size: 0.42mbFile type: pdf
We detail here the application of new column technology to purify histamine and serotonin (Table 1) in one extraction method with the elimination of the sorbent conditioning and equilibration steps familiar to traditional solid phase extraction (SPE) method protocols prior to measurements by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS). A secondary goal of combining two LC methods into one method was also achieved.
EVOLUTE EXPRESS cation exchange (CX), weak cation exchange (WCX) and acid-base-neutral (ABN) sorbents in a 96 well plate format prior to analysis by UPLC-MS/MS.