Part No: PPS451Issued year: 2017File size: 2.99mbFile type: pdf
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Part No: AN778Issued year: 2013File size: 0.88mbFile type: pdf
This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of salivary cortisol levels. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress. Analyte recoveries achieved using this method ranged from 96-99% with RSDs below 3% (n=7) for all analytes.
Cortisol, Saliva, SLE, Supported Liquid Extraction, ISOLUTE, Clinical, Steroid Hormones, Stress
Part No: AN777Issued year: 2013File size: 0.86mbFile type: pdf
Cortisol is a steroid hormone that in urine can be used to diagnose hyper or hypo cortisol diseases such as Cushing’s Syndrome. This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of urinary cortisol levels. Analyte recoveries achieved using this method ranged from 99-101% with RSDs below 5% for all analytes.
Cortisol, Steroid hormone, Cushing's Syndrome, Cushing's Disease, Clinical, SLE, Supported Liquid Extraction, Stress,
Part No: AN860Issued year: 2016File size: 0.89mbFile type: pdf
This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used.
High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.
Part No: AN762Issued year: 2012File size: 0.08mbFile type: pdf
This application note describes the extraction of testosterone and androstenedione from female patient serum samples using ISOLUTE® SLE+ (96 well) plates. This method has been optimized to extract testosterone and androstenedione from female clinical serum samples. The method has achieved very low limits of quantitation for testosterone and androstenedione 0.4nmol/L and 0.9nmol/L respectively. The recoveries for both analytes were greater than 90%.
Testosterone, Androstenedione, Clinical, Serum, ISOLUTE, SLE+, steroids,
Part No: AN740Issued year: 2011File size: 0.38mbFile type: pdf
This application note describes the extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ supported liquid extraction plates (96-well) with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% withb LOQs as low as 500 pg/mL.
ISOLUTE, TESTOSTERONE, SLE, SLE+, SUPPORTED LIQUID EXTRACTION, STEROID, HORMONE, PLASMA, ANDROGEN, CLINICAL
Part No: P145Issued year: 2016File size: 0.42mbFile type: pdf
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.
Part No: P055Issued year: 2013File size: 0.7mbFile type: pdf
Free urinary cortisol levels can be an indicator of a variety of disorders, such as Cushing Syndrome. This poster presents a simple method for the extraction of cortisol from urine demonstrating good recoveries and low ion suppression. LC-MS/MS analysis was performed using a Waters 2795 Liquid Handling Unit coupled to a Quattro Ultima Pt triple quadrupole MS using electrospray ionization, operated in the MRM mode. Supported liquid extraction method development resulted in recoveries greater than 90% for cortisol spiked urine. Calibration curves constructed using this method from 25-2000 ng/mL, demonstrated excellent linearity with coefficients of determination greater than 0.99.
Cortisol, Stress, Cushin's Syndrome, SLE, Supported Liquid Extraction, SPE, LLEMSACL, San Diego, 2013