Extraction of Estrone and Estradiol from Human Serum Using ISOLUTE® SLE+ Prior to HPLC-MS/MS (Without Derivatisation or Fluorine Adduct Stabilization)
Part No: AN860
Issued year: 2016
File size: 0.89mb
File type: pdf
This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used.
High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.
96-well plate
Application Notes
Clinical
English
Estradiol
Estrogens
Estrone
LC-MS/MS
Serum
Steroid Hormones