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Extraction of Estrone and Estradiol from Human Serum Using ISOLUTE® SLE+ Prior to HPLC-MS/MS (Without Derivatisation or Fluorine Adduct Stabilization)

Part No: AN860 Issued year: 2016 File size: 0.89mb File type: pdf

This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used. High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.

96-well plate Application Notes Clinical English Estradiol Estrogens Estrone LC-MS/MS Serum Steroid Hormones

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