This document compares automated sample preparation using the Biotage® Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.

96-well plate Clinical English Extrahera Forensic Indomethacin Ketoprofen LC-MS/MS NSAIDs Pharma Plasma Product Notes Sulindac

This poster evaluates the performance of a novel 96-well filter plate (ISOLUTE PLD+ Protein and Phospholipid Removal Plate) for the simultaneous removal of proteins and phospholipids from serum samples prior to LC-MS/MS analysis. ASMS2014

96-well plate Acidic Amitriptyline Analgesics Atenolol Basic Bretylium Clinical English Fluoxetine Forensic Ketoprofen LC-MS/MS Metoprolol Mianserin NSAIDs Naltrexone Pharma Plasma Posters Procainamide Quinidine Ranitidine Salbutamol Serum Sulindac Tricyclic Antidepressant β-blockers

This application note describes the use of the ISOLUTE® PLD+ Protein and Phospholipid Removal Plate for clean-up of a range of acidic, basic and neutral drugs from plasma. ISOLUTE® PLD+ Protein and Phospholipid Removal Plates are suitable for clean-up of a range of analytes with widely differing functionality and polarity characteristics from plasma, giving high recoveries, good reproducibility and excellent extract cleanliness.

96-well plate Acetaminophen Acidic Amitriptyline Application Notes Atenolol Basic Bretylium Brompheniramine Clinical English Fluoxetine Forensic Ketoprofen LC-MS/MS Metoprolol Mianserin NSAIDs Naltrexone Neutral Pharma Plasma Procainamide Quinidine Ranitidine Salbutamol Sulindac Tricyclic Antidepressant p-toluamide β-blockers

This Application Note highlights the use of supported-liquid Extraction to isolate three common NSAIDs, Sulindac, Flurbiprofen and Ibuprofen, from human plasma. Biotage, Ostro, Liquid liquid extraction, supported liquid extraction, Extrelute

Application Notes Clinical Flurbiprofen Ibuprofen LC-MS/MS NSAIDs Plasma Sulindac

Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase. molecularly imprinted polymers, MIP, BIOTAGE, AFFINILUTE, NSAIDs, Environmental, SupelMIP

Application Notes Clinical Clofibric Acid Column Ibuprofen LC-MS/MS NSAIDs Naproxen Post-Mortem fluids Post-Mortem tissue Water