Part No: P137Issued year: 2015File size: 0.37mbFile type: pdf
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
Part No: P137v2Issued year: 2016File size: 0.39mbFile type: pdf
This poster investigates various parts of the SPE method development process to develop a highly sensitive LC-MS/MS method for analysis of catecholamines (epinephrine, norepinephrine, dopamine).
A number of steps are optimized to improve sensitivity of the
analytes. This includes reducing the buffer strength in the mobile
phase, reducing the buffer strength of washes, careful pH control
throughout the extraction, avoiding an evaporation step and
incorporating IPA in the reconstitution solvent.
Part No: IST1071AIssued year: 2011File size: 0.17mbFile type: pdf
This method details the use of a non-polar retention mechanism for the extraction of catecholamines (noradrenaline, adrenaline, dopamine) from urine. The polar analytes form a non-polar complex with phenylboronic acid and are retained on the non-polar MFC18 column. Typical recoveries are >85%.
ISOLUTE, Catecholamines, dopind, sports drug testing,
Part No: AN844Issued year: 2015File size: 0.75mbFile type: pdf
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
Part No: AN871Issued year: 2017File size: 3.46mbFile type: pdf
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolites (metanephrine, normetanephrine and 3-methoxytyramine) from urine prior to LC-MS/MS detection.
Included in this application note are the detailed settings for implementing the method on the Biotage® Extrahera(TM) automation system.
Part No: P155Issued year: 2017File size: 0.61mbFile type: pdf
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Part No: P152Issued year: 2016File size: 0.49mbFile type: pdf
This poster describes a method for the simultaneous extraction (using EVOLUTE EXPRESS WCX) and analysis of plasma catecholamines and metanephrines. A highly sensitive, linear and rugged assay was developed, and automated using the Biotage Extrahera sample preparation processing system.
MSACL EU 2016