A Method for Automated Extraction of Pain Medication from Urine for Analysis by LC-MS-MS
Part No: P052
Issued year: 2013
File size: 0.48mb
File type: pdf
Pain management therapy warrants constant monitoring of therapeutic levels of prescribed drug levels in patient urine samples. The number of samples being submitted for analysis has increased dramatically in the last 10 years with improvements in high throughput automated screening capabilities. Patient samples analysis is complicated by the need for an effective sample preparation methodology that can extract target analytes from complex matrices with good efficiency. Further complicating the process is the need to enzymatically hydrolyse the glucoronidated metabolites prior to extraction from the urine matrix. A fully automated sample preparation process using a TECAN Freedom EVO® 100 was designed to incorporate both the enzymatic hydrolysis and subsequent sample preparation assay as one continuous workflow. Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) and solid phase extraction (SPE) techniques was used to extract a suite of pain management drugs from spiked urine samples. A recovery and quantitation assay was run on the TECAN Freedom EVO® 100 using mock patient samples to demonstrate utility of automation process.
MSACL, Pain Management, Biotage, SPE, SLE, LLE, Supported Liquid Extraction, Drugs, MSACL, San Diego, 2013
6-acetylmorphine (MAM)
96-well plate
Benzodiazepines
Clinical
Clonazepam
Codeine
Diazepam
Dihydrocodeine (DHC)
Drugs of Abuse
Flunitrazepam
Hydrocodone
Hydrolysed urine
Hydromorphone
LC-MS/MS
Methadone
Methamphetamine
Morphine
Nitrazepam
Opiates
Oxycodone
Oxymorphone
Posters
Urine