Part No: PPS443Issued year: 2017File size: 2.31mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: P156Issued year: 2017File size: 0.23mbFile type: pdf
Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds.
EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared.
MSACL 2017, Palm Springs
Part No: AN601Issued year: 2008File size: 0.12mbFile type: pdf
This Application Note describes the development of an automated procedure for high throughput supported-liquid extraction of three tricyclic antidepressant drugs from human plasma, using the ISOLUTE® SLE+ Supported-liquid Extraction Plate. Analyte recovery, along with the speed and efficiency are compared to traditional LLE.
Biotage, Extralute, Ostro, supported liquid extraction, liquid liquid extraction,
Part No: AN726Issued year: 2011File size: 0.17mbFile type: pdf
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation.
TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
Part No: AN727Issued year: 2011File size: 0.11mbFile type: pdf
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Part No: P165Issued year: 2017File size: 0.32mbFile type: pdf
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).