Part No: P116Issued year: 2014File size: 0.45mbFile type: pdf
This poster details current sample preparation strategies for aminoglycoside antibiotics in meat developed in a collaborative effort with Biotage and Tyson Foods, Inc. Springdale Corporate Laboratory to optimize analyte recovery (>70%), minimize analyte suppression and maintain acceptable method precision.
FAST LC-MS 2014
Whether working in the field of bio-analysis in
drug development, clinical or forensic analysis, or
with the diversity of samples in food safety and
environmental applications, the tools and
methods used for sample preparation have a
clear impact on productivity during method
development, validation and routine analysis.
EVOLUTE SPE sorbents and extraction
methodologies have been developed to extract a
wide range of analytes while reducing or
eliminating matrix components that can cause ion
suppression in LC-MS analysis.
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Part No: AN833Issued year: 2015File size: 1.33mbFile type: pdf
This application note describes a polymer based-based weak exchange mixed-mode SPE protocol for the extraction of the aminoglycoside antibiotics gentamicin, streptomycin, spectinomycin and neomycin from chicken meat prior to LC-MS/MS analysis. Linearity and LOQ meet USDA-FSIS guidelines.
Part No: P085Issued year: 2014File size: 0.46mbFile type: pdf
This poster demonstrates a novel, sensitive, cost effective and rugged method for the analysis of acrylamide in coffee and potato chips (crisps) using supported liquid extraction prior to LC-MS/MS
analysis. It demonstrates extraction from coffee down
to a concentration of 1 ng/mL and in potato chips to a level
of 10 ng/g (ppb).
Part No: AN796Issued year: 2013File size: 0.62mbFile type: pdf
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE® SLE+ columns with LC-MS/MS detection.
The method described in this application note achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppb in ground coffee (solid) or 125 ppb in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.
Part No: IST1076AIssued year: 2011File size: 0.12mbFile type: pdf
This method describes the clean-up of acrylamide from cooked foodstuffs, using a multimode retention mechanism (hydrophobic, cation and anion exchange) for retention of interfering food components. The analytical technique is LC-MS-MS.
ISOLUTE, MULTIMODE, SPE, FOOD, FOOD SCARE
Part No: AN807Issued year: 2013File size: 1.05mbFile type: pdf
This application note describes an SPE protocol appropriate for LC-MS/MS analysis of aflatoxin M1 found in infant formula.
The method described in this application note achieves high recoveries of aflatoxin M1 from infant formula with %RSDs and LOQs that all meet the requirements set in European regulations for its measurement.
Part No: AN785Issued year: 2014File size: 1.28mbFile type: pdf
This application note describes a solid phase extraction(SPE) protocol for the extraction of a range of mycotoxins from dried chili (pimiento) using ISOLUTE® Myco with LC-MS/MS analysis.
The method achieves high recoveries of aflatoxins and ochratoxin from dried chili (pimiento) with %RSDs and LOQs that meet the requirements set in European Union regulations for measurement of these analytes.
Part No: AN711Issued year: 2011File size: 0.11mbFile type: pdf
The following methods have been developed and optimized for the extraction of chloramphenicol from a variety of sample matrixes including milk, plasma, honey, urine, and shrimp/prawns for subsequent LC-MS/MS analysis. The methods are highly reproducible and offer low limits of detection.
AFFINILUTE, SupelMIP, MIP, Molecularly Imprinted Polymers,
Part No: AN714Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed and optimized for the extraction of fluoroquinolones (FQL) from a variety of sample matrixes including bovine kidney, honey, and milk. Example FQLs include sarafloxacin, norfloxacin, enrofloxacin, ciprofloxacin, lomefloxacin, and ofloxacin.
MIP, BIOTAGE, AFFINILUTE, SupelMIP, Molecularly Imprinted Polymers
Part No: AN765Issued year: 2012File size: 0.22mbFile type: pdf
Malachite green (MG) and crystal (gentian) violet (CV) are triphenylmethane dyes used in aquaculture as fungicides and ectoparasiticides. They are rapidly metabolised to the leucomalachite green (LMG) and leucocrystal (gentian) violet (LCV) metabolites that persist in fish tissue. MG and CV are banned in many countries due to their mutagenicity and carcinogenicity, but may still be used illegally. Monitoring methodologies must be able to detect the compounds at 1 ng/g (US) or 2 ng/g (EU). In order to meet these LODs for complex matrices such as oily fish, and the low UV absorbance of the leuco forms, LC-MS-MS is the analytical method of choice. This application note focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract. Additional cleanup is provided by polymeric EVOLUTE SPE columns, and the resulting extract demonstrated low ion suppression. Analyte recoveries of 60-105%, with low rsds, are achieved. The method is also applicable to Brilliant green.
Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Brilliant Green, EVOLUTE, EVOLUTE CX, QuEChERS, LC/MS/MS, Salmon, Contamination, Gentian violet, SPE, Solid Phase Extraction, Polymer, Resin,
Part No: AN733Issued year: 2011File size: 0.21mbFile type: pdf
This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible.
food scare, Melamine, China, kidney failure, renal failure, protein, Biotage, EVOLUTE,
Part No: AN732Issued year: 2011File size: 0.21mbFile type: pdf
This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Melamine's low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products.
Biotage, cation exchange, oasis, food scare, china,
Part No: AN749Issued year: 2011File size: 0.28mbFile type: pdf
Melamine is traditionally used in making plastics, however, it’s low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows recoveries of 88-90% with RSDs <10%.
Melamine, food scare, china, EVOLUTE, cation exchange, RapidTrace, Automation, SPE, sample prep,